Phytopathogen Effectors Use Multiple Mechanisms to Manipulate Plant Autophagy.
Identifieur interne : 000082 ( Main/Exploration ); précédent : 000081; suivant : 000083Phytopathogen Effectors Use Multiple Mechanisms to Manipulate Plant Autophagy.
Auteurs : Neeraj K. Lal [États-Unis] ; Burinrutt Thanasuwat [États-Unis] ; Pin-Jui Huang [États-Unis] ; Keri A. Cavanaugh [États-Unis] ; Amanda Carter [États-Unis] ; Richard W. Michelmore [États-Unis] ; Savithramma P. Dinesh-Kumar [États-Unis]Source :
- Cell host & microbe [ 1934-6069 ] ; 2020.
Abstract
Autophagy is a central part of immunity and hence is a key target of pathogens. However, the precise molecular mechanisms by which plant pathogens manipulate autophagy remain elusive. We identify a network of 88 interactions between 184 effectors from bacterial, fungal, oomycete, and nematode pathogens with 25 Arabidopsis autophagy (ATG) proteins. Notably, Pseudomonas syringae pv tomato (Pto) bacterial effectors HrpZ1, HopF3, and AvrPtoB employ distinct molecular strategies to modulate autophagy. Calcium-dependent HrpZ1 oligomerization targets ATG4b-mediated cleavage of ATG8 to enhance autophagy, while HopF3 also targets ATG8 but suppresses autophagy, with both effectors promoting infection. AvrPtoB affects ATG1 kinase phosphorylation and enhances bacterial virulence. Since pathogens inject limited numbers of effectors into hosts, our findings establish autophagy as a key target during infection. Additionally, as autophagy is enhanced and inhibited by these effectors, autophagy likely has different functions throughout infection and, thus, must be temporally and precisely regulated for successful infection.
DOI: 10.1016/j.chom.2020.07.010
PubMed: 32810441
Affiliations:
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Lal</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA. Electronic address: nkrlal@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Thanasuwat, Burinrutt" sort="Thanasuwat, Burinrutt" uniqKey="Thanasuwat B" first="Burinrutt" last="Thanasuwat">Burinrutt Thanasuwat</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Huang, Pin Jui" sort="Huang, Pin Jui" uniqKey="Huang P" first="Pin-Jui" last="Huang">Pin-Jui Huang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Cavanaugh, Keri A" sort="Cavanaugh, Keri A" uniqKey="Cavanaugh K" first="Keri A" last="Cavanaugh">Keri A. Cavanaugh</name><affiliation wicri:level="2"><nlm:affiliation>Departments of Plant Sciences, Molecular & Cellular Biology, Medical Microbiology & Immunology and the Genome Center, University of California, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Plant Sciences, Molecular & Cellular Biology, Medical Microbiology & Immunology and the Genome Center, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Carter, Amanda" sort="Carter, Amanda" uniqKey="Carter A" first="Amanda" last="Carter">Amanda Carter</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Michelmore, Richard W" sort="Michelmore, Richard W" uniqKey="Michelmore R" first="Richard W" last="Michelmore">Richard W. Michelmore</name><affiliation wicri:level="2"><nlm:affiliation>Departments of Plant Sciences, Molecular & Cellular Biology, Medical Microbiology & Immunology and the Genome Center, University of California, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Plant Sciences, Molecular & Cellular Biology, Medical Microbiology & Immunology and the Genome Center, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Dinesh Kumar, Savithramma P" sort="Dinesh Kumar, Savithramma P" uniqKey="Dinesh Kumar S" first="Savithramma P" last="Dinesh-Kumar">Savithramma P. Dinesh-Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA. Electronic address: spdineshkumar@ucdavis.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Cell host & microbe</title><idno type="eISSN">1934-6069</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Autophagy is a central part of immunity and hence is a key target of pathogens. However, the precise molecular mechanisms by which plant pathogens manipulate autophagy remain elusive. We identify a network of 88 interactions between 184 effectors from bacterial, fungal, oomycete, and nematode pathogens with 25 Arabidopsis autophagy (ATG) proteins. Notably, Pseudomonas syringae pv tomato (Pto) bacterial effectors HrpZ1, HopF3, and AvrPtoB employ distinct molecular strategies to modulate autophagy. Calcium-dependent HrpZ1 oligomerization targets ATG4b-mediated cleavage of ATG8 to enhance autophagy, while HopF3 also targets ATG8 but suppresses autophagy, with both effectors promoting infection. AvrPtoB affects ATG1 kinase phosphorylation and enhances bacterial virulence. Since pathogens inject limited numbers of effectors into hosts, our findings establish autophagy as a key target during infection. Additionally, as autophagy is enhanced and inhibited by these effectors, autophagy likely has different functions throughout infection and, thus, must be temporally and precisely regulated for successful infection.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32810441</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1934-6069</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>10</Month><Day>07</Day></PubDate></JournalIssue><Title>Cell host & microbe</Title><ISOAbbreviation>Cell Host Microbe</ISOAbbreviation></Journal><ArticleTitle>Phytopathogen Effectors Use Multiple Mechanisms to Manipulate Plant Autophagy.</ArticleTitle><Pagination><MedlinePgn>558-571.e6</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1931-3128(20)30406-6</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chom.2020.07.010</ELocationID><Abstract><AbstractText>Autophagy is a central part of immunity and hence is a key target of pathogens. However, the precise molecular mechanisms by which plant pathogens manipulate autophagy remain elusive. We identify a network of 88 interactions between 184 effectors from bacterial, fungal, oomycete, and nematode pathogens with 25 Arabidopsis autophagy (ATG) proteins. Notably, Pseudomonas syringae pv tomato (Pto) bacterial effectors HrpZ1, HopF3, and AvrPtoB employ distinct molecular strategies to modulate autophagy. Calcium-dependent HrpZ1 oligomerization targets ATG4b-mediated cleavage of ATG8 to enhance autophagy, while HopF3 also targets ATG8 but suppresses autophagy, with both effectors promoting infection. AvrPtoB affects ATG1 kinase phosphorylation and enhances bacterial virulence. Since pathogens inject limited numbers of effectors into hosts, our findings establish autophagy as a key target during infection. Additionally, as autophagy is enhanced and inhibited by these effectors, autophagy likely has different functions throughout infection and, thus, must be temporally and precisely regulated for successful infection.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lal</LastName><ForeName>Neeraj K</ForeName><Initials>NK</Initials><AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA. Electronic address: nkrlal@ucdavis.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thanasuwat</LastName><ForeName>Burinrutt</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Pin-Jui</ForeName><Initials>PJ</Initials><AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cavanaugh</LastName><ForeName>Keri A</ForeName><Initials>KA</Initials><AffiliationInfo><Affiliation>Departments of Plant Sciences, Molecular & Cellular Biology, Medical Microbiology & Immunology and the Genome Center, University of California, Davis, CA 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carter</LastName><ForeName>Amanda</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Michelmore</LastName><ForeName>Richard W</ForeName><Initials>RW</Initials><AffiliationInfo><Affiliation>Departments of Plant Sciences, Molecular & Cellular Biology, Medical Microbiology & Immunology and the Genome Center, University of California, Davis, CA 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dinesh-Kumar</LastName><ForeName>Savithramma P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA 95616, USA. Electronic address: spdineshkumar@ucdavis.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Cell Host Microbe</MedlineTA><NlmUniqueID>101302316</NlmUniqueID><ISSNLinking>1931-3128</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">ATG1</Keyword><Keyword MajorTopicYN="Y">ATG8</Keyword><Keyword MajorTopicYN="Y">Arabidopsis</Keyword><Keyword MajorTopicYN="Y">AvrPtoB</Keyword><Keyword MajorTopicYN="Y">HopF3</Keyword><Keyword MajorTopicYN="Y">HrpZ1</Keyword><Keyword MajorTopicYN="Y">Pseudomonas syringae</Keyword><Keyword MajorTopicYN="Y">autophagy</Keyword><Keyword MajorTopicYN="Y">effectors</Keyword><Keyword MajorTopicYN="Y">immunity</Keyword><Keyword MajorTopicYN="Y">pathogens</Keyword></KeywordList><CoiStatement>Declaration of Interests The authors declare no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32810441</ArticleId><ArticleId IdType="pii">S1931-3128(20)30406-6</ArticleId><ArticleId IdType="doi">10.1016/j.chom.2020.07.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Lal, Neeraj K" sort="Lal, Neeraj K" uniqKey="Lal N" first="Neeraj K" last="Lal">Neeraj K. Lal</name></region><name sortKey="Carter, Amanda" sort="Carter, Amanda" uniqKey="Carter A" first="Amanda" last="Carter">Amanda Carter</name><name sortKey="Cavanaugh, Keri A" sort="Cavanaugh, Keri A" uniqKey="Cavanaugh K" first="Keri A" last="Cavanaugh">Keri A. Cavanaugh</name><name sortKey="Dinesh Kumar, Savithramma P" sort="Dinesh Kumar, Savithramma P" uniqKey="Dinesh Kumar S" first="Savithramma P" last="Dinesh-Kumar">Savithramma P. Dinesh-Kumar</name><name sortKey="Huang, Pin Jui" sort="Huang, Pin Jui" uniqKey="Huang P" first="Pin-Jui" last="Huang">Pin-Jui Huang</name><name sortKey="Michelmore, Richard W" sort="Michelmore, Richard W" uniqKey="Michelmore R" first="Richard W" last="Michelmore">Richard W. Michelmore</name><name sortKey="Thanasuwat, Burinrutt" sort="Thanasuwat, Burinrutt" uniqKey="Thanasuwat B" first="Burinrutt" last="Thanasuwat">Burinrutt Thanasuwat</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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